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| 81. |  | SANTANA, R. C. M.; ZAFALON, L. F.; PILON, L. E.; TANAKA, E. V.; FELÍCIO, A. L. de AL; VESCHI, J. L. A.; BERGAMASCHI, M. A. C. M.; BRANDAO, H. de M. Sensibilidade a oxacilina de Staphylococcus coagulase-negativos isolados em infecções mamárias ovinas. Journal of Infection Control, v. 2, n. 1, p. 106, 2013. III CONGRESSO LATINO-AMERICANO DE RESISTÊNCIA MICROBIANA / X SUL ENCONTRO DE CONTROLE DE INFECÇÃO VII ENCONTRO GAÚCHO DE MICROBIOLOGIA / V JORNADA GAÚCHA DE ANTIMICROBIANOS APLICADA AO CONTROLE DE INFECÇÃO.| Biblioteca(s): Embrapa Pecuária Sudeste; Embrapa Semiárido. |
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| 82. | | GARCIA, A. R.; MACHADO, R.; ESTEVES, S. N.; REGITANO, L. C. de A.; NICIURA, S. C. M.; BERGAMASCHI, M. A. C. M.; SANTANA, R. C. M.; PEREIRA, V. S. do A. Contribuição atual da Embrapa Pecuária Sudeste ao desenvolvimento da andrologia de ruminantes de interesse zootécnico. In: REUNIÃO DE ANDROLOGIA ANIMAL, 1., 2016, São Paulo. Anais... São Paulo: [s.n.], 2016.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
29/11/2012 |
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Data da última atualização: |
18/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LIU, J.; LUO, X.; SHAFF, J.; LIANG, C.; JIA, X.; LI, Z.; MAGALHAES, J.; KOCHIAN, L. V. |
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Afiliação: |
Jiping Liu, Cornell University, Ithaca, NY.; Xiaoying Luo, Southwest University, CHINA.; Jon Shaff, Cornell University, USA; Cuiyue Liang, South China Agriculture University, China.; Xiaomin Jia, Cornell University, USA.; Ziyan Li, Northwest Sci-Tech University of Agriculture and Forestry, China; JURANDIR VIEIRA DE MAGALHAES, CNPMS; Leon V. Kochian, Cornell University, USA. |
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Título: |
A promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
The Plant Journal, Oxford, v. 71, p. 327-337, 2012. |
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DOI: |
10.1111/j.1365-313X.2012.04994.x |
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Idioma: |
Inglês |
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Conteúdo: |
The primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance. MenosThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only sig... Mostrar Tudo |
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Palavras-Chave: |
Aluminum resistance; Carbon-use efficiency; Organic acid exudation; Promoter swap; Transporter protein. |
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Thesaurus NAL: |
Arabidopsis thaliana. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02528naa a2200289 a 4500
001 1940816
005 2017-05-18
008 2012 bl uuuu u00u1 u #d
024 7 $a10.1111/j.1365-313X.2012.04994.x$2DOI
100 1 $aLIU, J.
245 $aA promoter-swap strategy between the AtALMT and AtMATE genes increased Arabidopsis aluminum resistance and improved carbon-use efficiency for aluminum resistance.$h[electronic resource]
260 $c2012
520 $aThe primary mechanism of Arabidopsis aluminum (Al) resistance is based on root Al exclusion, resulting from Al-activated root exudation of the Al3+-chelating organic acids, malate and citrate. Root malate exudation is the major contributor to Arabidopsis Al resistance, and is conferred by expression of AtALMT1, which encodes the root malate transporter. Root citrate exudation plays a smaller but still significant role in Arabidopsis Al resistance, and is conferred by expression of AtMATE, which encodes the root citrate transporter. In this study, we demonstrate that levels of Al-activated root organic acid exudation are closely correlated with expression of the organic acid transporter genes AtALMT1 and AtMATE. We also found that the AtALMT1 promoter confers a significantly higher level of gene expression than the AtMATE promoter. Analysis of AtALMT1 and AtMATE tissue- and cell-specific expression based on stable expression of promoter–reporter gene constructs showed that the two genes are expressed incomplementary root regions: AtALMT1 is expressed in the root apices, while AtMATE is expressed in the mature portions of the roots. As citrate is a much more effective chelator of Al3+ than malate, we used a promoter-swap strategy to test whether root tip-localized expression of the AtMATE coding region driven by the stronger AtALMT1 promoter (AtALMT1P::AtMATE) resulted in increased Arabidopsis Al resistance. Our results indicate that expression of AtALMT1P::AtMATE not only significantly increased Al resistance of the transgenic plants, but also enhanced carbon-use efficiency for Al resistance.
650 $aArabidopsis thaliana
653 $aAluminum resistance
653 $aCarbon-use efficiency
653 $aOrganic acid exudation
653 $aPromoter swap
653 $aTransporter protein
700 1 $aLUO, X.
700 1 $aSHAFF, J.
700 1 $aLIANG, C.
700 1 $aJIA, X.
700 1 $aLI, Z.
700 1 $aMAGALHAES, J.
700 1 $aKOCHIAN, L. V.
773 $tThe Plant Journal, Oxford$gv. 71, p. 327-337, 2012.
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